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Evidence for proton transfer in the rate-limiting step of a fast-cleaving Varkud satellite ribozyme
A fast-cleaving version of the Varkud satellite ribozyme, called RG, shows an apparent cis-cleavage rate constant of 5 sec(−1), similar to the rates of protein enzymes that catalyze similar reactions. Here, we describe mutational, pH-rate, and kinetic solvent isotope experiments that investigate the...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1851575/ https://ncbi.nlm.nih.gov/pubmed/17389378 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0608864104 |
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