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A two-metal ion mechanism operates in the hammerhead ribozyme-mediated cleavage of an RNA substrate

Evidence for a two-metal ion mechanism for cleavage of the HH16 hammerhead ribozyme is provided by monitoring the rate of cleavage of the RNA substrate as a function of La(3+) concentration in the presence of a constant concentration of Mg(2+). We show that a bell-shaped curve of cleavage activation...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Lott, William B., Pontius, Brian W., von Hippel, Peter H.
Format: Artigo
Sprache:Inglês
Veröffentlicht: The National Academy of Sciences 1998
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC18456/
https://ncbi.nlm.nih.gov/pubmed/9435228
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