Dwell-Time Distribution Analysis of Polyprotein Unfolding Using Force-Clamp Spectroscopy

Using the recently developed single molecule force-clamp technique we quantitatively measure the kinetics of conformational changes of polyprotein molecules at a constant force. In response to an applied force of 110 pN, we measure the dwell times of 1647 unfolding events of individual ubiquitin mod...

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Detaylı Bibliyografya
Asıl Yazarlar: Brujić, Jasna, Hermans, Rodolfo I. Z., Garcia-Manyes, Sergi, Walther, Kirstin A., Fernandez, Julio M.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: The Biophysical Society 2007
Konular:
Proteins
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC1831696/
https://ncbi.nlm.nih.gov/pubmed/17259284
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.099481
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