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A system to generate chromosomal mutations in Lactococcus lactis which allows fast analysis of targeted genes.

A system for generating chromosomal insertions in lactococci is described. It is based on the conditional replication of lactococcal pWV01-derived Ori+ RepA- vector pORI19, containing lacZ alpha and the multiple cloning site of pUC19. Chromosomal AluI fragments of Lactococcus lactis were cloned in p...

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Bibliografiske detaljer
Main Authors: Law, J, Buist, G, Haandrikman, A, Kok, J, Venema, G, Leenhouts, K
Format: Artigo
Sprog:Inglês
Udgivet: 1995
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC177576/
https://ncbi.nlm.nih.gov/pubmed/8522504
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