A system to generate chromosomal mutations in Lactococcus lactis which allows fast analysis of targeted genes.
A system for generating chromosomal insertions in lactococci is described. It is based on the conditional replication of lactococcal pWV01-derived Ori+ RepA- vector pORI19, containing lacZ alpha and the multiple cloning site of pUC19. Chromosomal AluI fragments of Lactococcus lactis were cloned in p...
Zapisane w:
| Wydane w: | J Bacteriol |
|---|---|
| Główni autorzy: | , , , , , |
| Format: | Artigo |
| Język: | Inglês |
| Wydane: |
American Society for Microbiology (ASM)
1995
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| Hasła przedmiotowe: | |
| Dostęp online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC177576/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8522504/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.177.24.7011-7018.1995 |
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