A system to generate chromosomal mutations in Lactococcus lactis which allows fast analysis of targeted genes.

A system for generating chromosomal insertions in lactococci is described. It is based on the conditional replication of lactococcal pWV01-derived Ori+ RepA- vector pORI19, containing lacZ alpha and the multiple cloning site of pUC19. Chromosomal AluI fragments of Lactococcus lactis were cloned in p...

Fuld beskrivelse

Bibliografiske detaljer

Udgivet i:	J Bacteriol
Principais autores:	Law, J, Buist, G, Haandrikman, A, Kok, J, Venema, G, Leenhouts, K
Format:	Artigo
Sprog:	Inglês
Udgivet:	American Society for Microbiology (ASM) 1995
Fag:	Research Article
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC177576/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8522504/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.177.24.7011-7018.1995
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