Studies on the activity of the hypoxia-inducible-factor hydroxylases using an oxygen consumption assay

The activity and levels of the metazoan HIF (hypoxia-inducible factor) are regulated by its hydroxylation, catalysed by 2OG (2-oxoglutarate)- and Fe(II)-dependent dioxygenases. An oxygen consumption assay was developed and used to study the relationship between HIF hydroxylase activity and oxygen co...

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Main Authors: Ehrismann, Dominic, Flashman, Emily, Genn, David N., Mathioudakis, Nicolas, Hewitson, Kirsty S., Ratcliffe, Peter J., Schofield, Christopher J.
Formato: Artigo
Idioma:Inglês
Publicado: Portland Press Ltd. 2006
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Research Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC1698668/
https://ncbi.nlm.nih.gov/pubmed/16952279
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20061151
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