Use of a fluorogenic probe in a PCR-based assay for the detection of Listeria monocytogenes.

A PCR-based assay for Listeria monocytogenes that uses the hydrolysis of an internal fluorogenic probe to monitor the amplification of the target has been formatted. The fluorogenic 5' nuclease PCR assay takes advantage of the endogenous 5' --> 3' nuclease activity of Taq DNA polymerase to digest a...

ver descrição completa

Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Bassler, H A, Flood, S J, Livak, K J, Marmaro, J, Knorr, R, Batt, C A
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1995
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167671/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7487008/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.61.10.3724-3728.1995
Tags:	Adicionar Tag Sem tags, seja o primeiro a adicionar uma tag!