Use of a fluorogenic probe in a PCR-based assay for the detection of Listeria monocytogenes.

A PCR-based assay for Listeria monocytogenes that uses the hydrolysis of an internal fluorogenic probe to monitor the amplification of the target has been formatted. The fluorogenic 5' nuclease PCR assay takes advantage of the endogenous 5' --> 3' nuclease activity of Taq DNA polymerase to digest a...

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書誌詳細

出版年:	Appl Environ Microbiol
主要な著者:	Bassler, H A, Flood, S J, Livak, K J, Marmaro, J, Knorr, R, Batt, C A
フォーマット:	Artigo
言語:	Inglês
出版事項:	American Society for Microbiology (ASM) 1995
主題:	Research Article
オンライン･アクセス:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167671/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7487008/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.61.10.3724-3728.1995
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