Use of a fluorogenic probe in a PCR-based assay for the detection of Listeria monocytogenes.

A PCR-based assay for Listeria monocytogenes that uses the hydrolysis of an internal fluorogenic probe to monitor the amplification of the target has been formatted. The fluorogenic 5' nuclease PCR assay takes advantage of the endogenous 5' --> 3' nuclease activity of Taq DNA polymerase to digest a...

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Bibliografski detalji

Izdano u:	Appl Environ Microbiol
Glavni autori:	Bassler, H A, Flood, S J, Livak, K J, Marmaro, J, Knorr, R, Batt, C A
Format:	Artigo
Jezik:	Inglês
Izdano:	American Society for Microbiology (ASM) 1995
Teme:	Research Article
Online pristup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167671/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7487008/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.61.10.3724-3728.1995
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