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Development of a Fluorogenic 5′ Nuclease PCR Assay for Detection of the ail Gene of Pathogenic Yersinia enterocolitica

In this report we describe the development and evaluation of a fluorogenic PCR assay for the detection of pathogenic Yersinia enterocolitica. The assay targets the chromosomally encoded attachment and invasion gene, ail. Three primer-probe sets (TM1, TM2, and TM3) amplifying different, yet overlappi...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Jourdan, Alissa D., Johnson, Scott C., Wesley, Irene V.
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2000
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC92216/
https://ncbi.nlm.nih.gov/pubmed/10966386
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