Development of a Fluorogenic 5′ Nuclease PCR Assay for Detection of the ail Gene of Pathogenic Yersinia enterocolitica

In this report we describe the development and evaluation of a fluorogenic PCR assay for the detection of pathogenic Yersinia enterocolitica. The assay targets the chromosomally encoded attachment and invasion gene, ail. Three primer-probe sets (TM1, TM2, and TM3) amplifying different, yet overlappi...

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Detalles Bibliográficos

Publicado en:	Appl Environ Microbiol
Autores principales:	Jourdan, Alissa D., Johnson, Scott C., Wesley, Irene V.
Formato:	Artigo
Lenguaje:	Inglês
Publicado:	American Society for Microbiology (ASM) 2000
Materias:	Food Microbiology
Acceso en línea:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC92216/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10966386/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.66.9.3750-3755.2000
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