Advancing uracil-excision based cloning towards an ideal technique for cloning PCR fragments

The largely unused uracil-excision molecular cloning technique has excellent features in most aspects compared to other modern cloning techniques. Its application has, however, been hampered by incompatibility with proof-reading DNA polymerases. We have advanced the technique by identifying PfuCx as...

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Main Authors: Nour-Eldin, Hussam H., Hansen, Bjarne G., Nørholm, Morten H. H., Jensen, Jacob K., Halkier, Barbara A.
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2006
Fag:
Methods Online
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1635280/
https://ncbi.nlm.nih.gov/pubmed/17000637
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkl635
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