USER fusion: a rapid and efficient method for simultaneous fusion and cloning of multiple PCR products

We present a method that allows simultaneous fusion and cloning of multiple PCR products in a rapid and efficient manner. The procedure is based on the use of PCR primers that contain a single deoxyuridine residue near their 5′ end. Treatment of the PCR products with a commercial deoxyuridine-excisi...

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Bibliographic Details
Main Authors: Geu-Flores, Fernando, Nour-Eldin, Hussam H., Nielsen, Morten T., Halkier, Barbara A.
Format: Artigo
Language:Inglês
Published: Oxford University Press 2007
Subjects:
Methods Online
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1874642/
https://ncbi.nlm.nih.gov/pubmed/17389646
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkm106
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