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Advancing uracil-excision based cloning towards an ideal technique for cloning PCR fragments
The largely unused uracil-excision molecular cloning technique has excellent features in most aspects compared to other modern cloning techniques. Its application has, however, been hampered by incompatibility with proof-reading DNA polymerases. We have advanced the technique by identifying PfuCx as...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2006
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1635280/ https://ncbi.nlm.nih.gov/pubmed/17000637 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkl635 |
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