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USER™ friendly DNA engineering and cloning method by uracil excision
Here we report a PCR-based DNA engineering technique for seamless assembly of recombinant molecules from multiple components. We create cloning vector and target molecules flanked with compatible single-stranded (ss) extensions. The vector contains a cassette with two inversely oriented nicking endo...
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| Main Authors: | , , , , , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Oxford University Press
2007
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1874603/ https://ncbi.nlm.nih.gov/pubmed/17341463 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkm041 |
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