UCE: A uracil excision (USER™)-based toolbox for transformation of cereals

BACKGROUND: Cloning of gene casettes and other DNA sequences into the conventional vectors for biolistic or Agrobacterium-mediated transformation is hampered by a limited amount of unique restriction sites and by the difficulties often encountered when ligating small single strand DNA overhangs. The...

पूर्ण विवरण

में बचाया:
ग्रंथसूची विवरण
मुख्य लेखकों: Hebelstrup, Kim H, Christiansen, Michael W, Carciofi, Massimiliano, Tauris, Birgitte, Brinch-Pedersen, Henrik, Holm, Preben B
स्वरूप: Artigo
भाषा:Inglês
प्रकाशित: BioMed Central 2010
विषय:
Methodology
ऑनलाइन पहुंच:https://ncbi.nlm.nih.gov/pmc/articles/PMC2892451/
https://ncbi.nlm.nih.gov/pubmed/20537147
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1746-4811-6-15
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