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A mutant Pfu DNA polymerase designed for advanced uracil-excision DNA engineering
BACKGROUND: The combined use of restriction enzymes with PCR has revolutionized molecular cloning, but is inherently restricted by the content of the manipulated DNA sequences. Uracil-excision based cloning is ligase and sequence independent and allows seamless fusion of multiple DNA sequences in si...
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| Hlavní autor: | |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
BioMed Central
2010
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2847956/ https://ncbi.nlm.nih.gov/pubmed/20233396 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-10-21 |
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