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A mutant Pfu DNA polymerase designed for advanced uracil-excision DNA engineering

BACKGROUND: The combined use of restriction enzymes with PCR has revolutionized molecular cloning, but is inherently restricted by the content of the manipulated DNA sequences. Uracil-excision based cloning is ligase and sequence independent and allows seamless fusion of multiple DNA sequences in si...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile nagusia: Nørholm, Morten HH
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: BioMed Central 2010
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC2847956/
https://ncbi.nlm.nih.gov/pubmed/20233396
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-10-21
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