Consensus-Degenerate Hybrid Oligonucleotide Primers for Amplification of Priming Glycosyltransferase Genes of the Exopolysaccharide Locus in Strains of the Lactobacillus casei Group

A primer design strategy named CODEHOP (consensus-degenerate hybrid oligonucleotide primer) for amplification of distantly related sequences was used to detect the priming glycosyltransferase (GT) gene in strains of the Lactobacillus casei group. Each hybrid primer consisted of a short 3′ degenerate...

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Main Authors: Provencher, Cathy, LaPointe, Gisèle, Sirois, Stéphane, Van Calsteren, Marie-Rose, Roy, Denis
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 2003
Teme:
Food Microbiology
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC161484/
https://ncbi.nlm.nih.gov/pubmed/12788729
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.6.3299-3307.2003
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