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Consensus-Degenerate Hybrid Oligonucleotide Primers for Amplification of Priming Glycosyltransferase Genes of the Exopolysaccharide Locus in Strains of the Lactobacillus casei Group

A primer design strategy named CODEHOP (consensus-degenerate hybrid oligonucleotide primer) for amplification of distantly related sequences was used to detect the priming glycosyltransferase (GT) gene in strains of the Lactobacillus casei group. Each hybrid primer consisted of a short 3′ degenerate...

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Detalhes bibliográficos
Publicado no:Appl Environ Microbiol
Principais autores: Provencher, Cathy, LaPointe, Gisèle, Sirois, Stéphane, Van Calsteren, Marie-Rose, Roy, Denis
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2003
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC161484/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12788729/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.69.6.3299-3307.2003
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