Quantitative analysis of the effect of the mutation frequency on the affinity maturation of single chain Fv antibodies

Random mutagenesis and selection using phage or cell surface display provides an efficient method for affinity maturation of single chain Fv (scFv) antibodies, thereby improving function in various applications. To investigate the effects of mutation frequency on affinity maturation, error-prone PCR...

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Библиографические подробности

Опубликовано в::	Proc Natl Acad Sci U S A
Главные авторы:	Daugherty, Patrick S., Chen, Gang, Iverson, Brent L., Georgiou, George
Формат:	Artigo
Язык:	Inglês
Опубликовано:	National Academy of Sciences 2000
Предметы:	Biological Sciences
Online-ссылка:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC15748/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10688877/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.030527597
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