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Quantitative analysis of the effect of the mutation frequency on the affinity maturation of single chain Fv antibodies

Random mutagenesis and selection using phage or cell surface display provides an efficient method for affinity maturation of single chain Fv (scFv) antibodies, thereby improving function in various applications. To investigate the effects of mutation frequency on affinity maturation, error-prone PCR...

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Autors principals: Daugherty, Patrick S., Chen, Gang, Iverson, Brent L., Georgiou, George
Format: Artigo
Idioma:Inglês
Publicat: The National Academy of Sciences 2000
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC15748/
https://ncbi.nlm.nih.gov/pubmed/10688877
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