Quantitative analysis of the effect of the mutation frequency on the affinity maturation of single chain Fv antibodies

Random mutagenesis and selection using phage or cell surface display provides an efficient method for affinity maturation of single chain Fv (scFv) antibodies, thereby improving function in various applications. To investigate the effects of mutation frequency on affinity maturation, error-prone PCR...

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Veröffentlicht in:	Proc Natl Acad Sci U S A
Hauptverfasser:	Daugherty, Patrick S., Chen, Gang, Iverson, Brent L., Georgiou, George
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	National Academy of Sciences 2000
Schlagworte:	Biological Sciences
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC15748/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10688877/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.030527597
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