Quantitative analysis of the effect of the mutation frequency on the affinity maturation of single chain Fv antibodies

Random mutagenesis and selection using phage or cell surface display provides an efficient method for affinity maturation of single chain Fv (scFv) antibodies, thereby improving function in various applications. To investigate the effects of mutation frequency on affinity maturation, error-prone PCR...

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Pubblicato in:	Proc Natl Acad Sci U S A
Autori principali:	Daugherty, Patrick S., Chen, Gang, Iverson, Brent L., Georgiou, George
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	National Academy of Sciences 2000
Soggetti:	Biological Sciences
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC15748/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10688877/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.030527597
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