Enzymatic Regional Methylation Assay: A Novel Method to Quantify Regional CpG Methylation Density

We have developed a novel quantitative method for rapidly assessing the CpG methylation density of a DNA region in mammalian cells. After bisulfite modification of genomic DNA, the region of interest is PCR amplified with primers containing two dam sites (GATC). The purified PCR products are then in...

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Autori principali: Galm, Oliver, Rountree, Michael R., Bachman, Kurtis E., Jair, Kam-Wing, Baylin, Stephen B., Herman, James G.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Cold Spring Harbor Laboratory Press 2002
Soggetti:
Methods
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC155259/
https://ncbi.nlm.nih.gov/pubmed/11779840
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.202501
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