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Enzymatic Regional Methylation Assay: A Novel Method to Quantify Regional CpG Methylation Density
We have developed a novel quantitative method for rapidly assessing the CpG methylation density of a DNA region in mammalian cells. After bisulfite modification of genomic DNA, the region of interest is PCR amplified with primers containing two dam sites (GATC). The purified PCR products are then in...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Cold Spring Harbor Laboratory Press
2002
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC155259/ https://ncbi.nlm.nih.gov/pubmed/11779840 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.202501 |
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