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Enzymatic Regional Methylation Assay: A Novel Method to Quantify Regional CpG Methylation Density

We have developed a novel quantitative method for rapidly assessing the CpG methylation density of a DNA region in mammalian cells. After bisulfite modification of genomic DNA, the region of interest is PCR amplified with primers containing two dam sites (GATC). The purified PCR products are then in...

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Udgivet i:Genome Res
Principais autores: Galm, Oliver, Rountree, Michael R., Bachman, Kurtis E., Jair, Kam-Wing, Baylin, Stephen B., Herman, James G.
Format: Artigo
Sprog:Inglês
Udgivet: Cold Spring Harbor Laboratory Press 2002
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Methods
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC155259/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11779840/
https://ncbi.nlm.nih.govhttps://doi.org/10.1101/gr.202501
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