Enzymatic Regional Methylation Assay: A Novel Method to Quantify Regional CpG Methylation Density

We have developed a novel quantitative method for rapidly assessing the CpG methylation density of a DNA region in mammalian cells. After bisulfite modification of genomic DNA, the region of interest is PCR amplified with primers containing two dam sites (GATC). The purified PCR products are then in...

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Bibliografische gegevens
Hoofdauteurs: Galm, Oliver, Rountree, Michael R., Bachman, Kurtis E., Jair, Kam-Wing, Baylin, Stephen B., Herman, James G.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Cold Spring Harbor Laboratory Press 2002
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC155259/
https://ncbi.nlm.nih.gov/pubmed/11779840
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.202501
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