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The integrity of a cholesterol-binding pocket in Niemann–Pick C2 protein is necessary to control lysosome cholesterol levels

The neurodegenerative disease Niemann–Pick Type C2 (NPC2) results from mutations in the NPC2 (HE1) gene that cause abnormally high cholesterol accumulation in cells. We find that purified NPC2, a secreted soluble protein, binds cholesterol specifically with a much higher affinity (K(d) = 30–50 nM) t...

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Detalhes bibliográficos
Main Authors: Ko, Dennis C., Binkley, Jonathan, Sidow, Arend, Scott, Matthew P.
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences 2003
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC151373/
https://ncbi.nlm.nih.gov/pubmed/12591949
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0530027100
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