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Discovery of aminoacyl-tRNA synthetase activity through cell-surface display of noncanonical amino acids

The incorporation of noncanonical amino acids into recombinant proteins in Escherichia coli can be facilitated by the introduction of new aminoacyl-tRNA synthetase activity into the expression host. We describe here a screening procedure for the identification of new aminoacyl-tRNA synthetase activi...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Link, A. James, Vink, Mandy K. S., Agard, Nicholas J., Prescher, Jennifer A., Bertozzi, Carolyn R., Tirrell, David A.
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 2006
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1502431/
https://ncbi.nlm.nih.gov/pubmed/16801548
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0601167103
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