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Enzymatic mutation detection. Procedure for screening and mapping of mutations by immobilised endonuclease VII.

Endonuclease VII (endo VII) binds to non-pairing nucleotides in DNA. This served as the basis for the development of a mutation detection assay involving immobilised endo VII and heteroduplex DNAs made by hybridisation of mutant and wild type DNA. The use of microtiter plates allows screening of lar...

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Udgivet i:Nucleic Acids Res
Principais autores: Golz, S, Birkenkamp-Demtröder, K, Kemper, B
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1998
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147345/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9461481/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.4.1132
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