A new quantitative (two-photon extracellular polar-tracer imaging-based quantification (TEPIQ)) analysis for diameters of exocytic vesicles and its application to mouse pancreatic islets

We have developed an imaging approach to estimate the diameter of exocytic vesicles that are smaller than the resolution of an optical microscope and present within intact tissue. This approach is based on two-photon excitation imaging of polar tracers in the extracellular medium, is designated TEPI...

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Detalhes bibliográficos
Main Authors: Kasai, Haruo, Hatakeyama, Hiroyasu, Kishimoto, Takuya, Liu, Ting-Ting, Nemoto, Tomomi, Takahashi, Noriko
Formato: Artigo
Idioma:Inglês
Publicado em: Blackwell Science Inc 2005
Assuntos:
Cell Physiology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1464182/
https://ncbi.nlm.nih.gov/pubmed/16150799
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1113/jphysiol.2005.093047
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