A new quantitative (two-photon extracellular polar-tracer imaging-based quantification (TEPIQ)) analysis for diameters of exocytic vesicles and its application to mouse pancreatic islets

We have developed an imaging approach to estimate the diameter of exocytic vesicles that are smaller than the resolution of an optical microscope and present within intact tissue. This approach is based on two-photon excitation imaging of polar tracers in the extracellular medium, is designated TEPI...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Kasai, Haruo, Hatakeyama, Hiroyasu, Kishimoto, Takuya, Liu, Ting-Ting, Nemoto, Tomomi, Takahashi, Noriko
Format: Artigo
Sprache:Inglês
Veröffentlicht: Blackwell Science Inc 2005
Schlagworte:
Cell Physiology
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1464182/
https://ncbi.nlm.nih.gov/pubmed/16150799
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1113/jphysiol.2005.093047
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