A new quantitative (two-photon extracellular polar-tracer imaging-based quantification (TEPIQ)) analysis for diameters of exocytic vesicles and its application to mouse pancreatic islets

We have developed an imaging approach to estimate the diameter of exocytic vesicles that are smaller than the resolution of an optical microscope and present within intact tissue. This approach is based on two-photon excitation imaging of polar tracers in the extracellular medium, is designated TEPI...

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Hlavní autoři: Kasai, Haruo, Hatakeyama, Hiroyasu, Kishimoto, Takuya, Liu, Ting-Ting, Nemoto, Tomomi, Takahashi, Noriko
Médium: Artigo
Jazyk:Inglês
Vydáno: Blackwell Science Inc 2005
Témata:
Cell Physiology
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1464182/
https://ncbi.nlm.nih.gov/pubmed/16150799
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1113/jphysiol.2005.093047
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