Structure-function analysis of yeast mRNA cap methyltransferase and high-copy suppression of conditional mutants by AdoMet synthase and the ubiquitin conjugating enzyme Cdc34p.

Here we present a genetic analysis of the yeast cap-methylating enzyme Abd1p. To identify individual amino acids required for Abd1p function, we introduced alanine mutations at 35 positions of the 436-amino acid yeast protein. Two new recessive lethal mutations, F256A and Y330A, were identified. All...

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Detalhes bibliográficos
Main Authors: Schwer, B, Saha, N, Mao, X, Chen, H W, Shuman, S
Formato: Artigo
Idioma:Inglês
Publicado em: 2000
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Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1461192/
https://ncbi.nlm.nih.gov/pubmed/10924457
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