Hypermutagenic PCR involving all four transitions and a sizeable proportion of transversions.

Very complex mutant libraries of the dihydrofolate reductase (DHFR) gene encoded by the Escherichia coli plasmid R67 were created using hypermutagenic PCR with biased deoxynucleotide triphosphate (dNTP) concentrations. Exploiting the particular stability of the G:T mismatch, the DHFR gene could be e...

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Main Authors: Vartanian, J P, Henry, M, Wain-Hobson, S
Format: Artigo
Sprog:Inglês
Udgivet: 1996
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC145995/
https://ncbi.nlm.nih.gov/pubmed/8758987
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