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Strand specific PCR amplification of low copy number DNA.

A method for the amplification of a single DNA strand at low copy number is described. It is a wholly PCR based approach which involves an initial linear amplification of the target using a tagged strand specific primer. This is followed by classical PCR amplification of the progeny using a pair of...

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Bibliographic Details
Main Authors: Meyerhans, A, Vartanian, J P, Wain-Hobson, S
Format: Artigo
Language:Inglês
Published: 1992
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC310417/
https://ncbi.nlm.nih.gov/pubmed/1741286
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