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Improved DOP-PCR (iDOP-PCR): A robust and simple WGA method for efficient amplification of low copy number genomic DNA

Whole-genome amplification (WGA) techniques are used for non-specific amplification of low-copy number DNA, and especially for single-cell genome and transcriptome amplification. There are a number of WGA methods that have been developed over the years. One example is degenerate oligonucleotide-prim...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Blagodatskikh, Konstantin A., Kramarov, Vladimir M., Barsova, Ekaterina V., Garkovenko, Alexey V., Shcherbo, Dmitriy S., Shelenkov, Andrew A., Ustinova, Vera V., Tokarenko, Maria R., Baker, Simon C., Kramarova, Tatiana V., Ignatov, Konstantin B.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5593185/
https://ncbi.nlm.nih.gov/pubmed/28892497
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0184507
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