A high-throughput assay for quantitative measurement of PCR errors

The accuracy with which DNA polymerase can replicate a template DNA sequence is an extremely important property that can vary by an order of magnitude from one enzyme to another. The rate of nucleotide misincorporation is shaped by multiple factors, including PCR conditions and proofreading capabili...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Shagin, Dmitriy A., Shagina, Irina A., Zaretsky, Andrew R., Barsova, Ekaterina V., Kelmanson, Ilya V., Lukyanov, Sergey, Chudakov, Dmitriy M., Shugay, Mikhail
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2017
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5457411/
https://ncbi.nlm.nih.gov/pubmed/28578414
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-02727-8
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