Application of nonsense-mediated primer exclusion (NOPE) for preparation of unique molecular barcoded libraries

BACKGROUND: Recently we proposed efficient method to exclude undesirable primers at any stage of amplification reaction, here termed NOPE (NOnsense-mediated Primer Exclusion). According to this method, added oligonucleotide overlapping with the 3′-end of unwanted amplification primer (NOPE oligo) si...

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Detalhes bibliográficos
Publicado no:BMC Genomics
Main Authors: Shagin, Dmitriy A., Turchaninova, Maria A., Shagina, Irina A., Shugay, Mikhail, Zaretsky, Andrew R., Zueva, Olga I., Bolotin, Dmitriy A., Lukyanov, Sergey, Chudakov, Dmitriy M.
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2017
Assuntos:
Methodology Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5460480/
https://ncbi.nlm.nih.gov/pubmed/28583065
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12864-017-3815-2
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