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Probing a protein–protein interaction by in vitro evolution

In this study, we used in vitro protein evolution with ribosome and phage display to optimize the affinity of a human IL-13-neutralizing antibody, a therapeutic candidate for the treatment of asthma, >150-fold to 81 pM by using affinity-driven stringency selections. Simultaneously, the antibody p...

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Hlavní autoři: Thom, George, Cockroft, Alexis C., Buchanan, Andrew G., Joberty Candotti, Cathy, Cohen, E. Suzanne, Lowne, David, Monk, Phill, Shorrock-Hart, Celia P., Jermutus, Lutz, Minter, Ralph R.
Médium: Artigo
Jazyk:Inglês
Vydáno: National Academy of Sciences 2006
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1458619/
https://ncbi.nlm.nih.gov/pubmed/16684878
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0602341103
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