A helicase assay based on the displacement of fluorescent, nucleic acid-binding ligands.

We have developed a new helicase assay that overcomes many limitations of other assays used to measure this activity. This continuous, kinetic assay is based on the displacement of fluorescent dyes from dsDNA upon DNA unwinding. These ligands exhibit significant fluorescence enhancement when bound t...

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Bibliografische Detailangaben

Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Eggleston, A K, Rahim, N A, Kowalczykowski, S C
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 1996
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC145774/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8614617/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/24.7.1179
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