A helicase assay based on the displacement of fluorescent, nucleic acid-binding ligands.

We have developed a new helicase assay that overcomes many limitations of other assays used to measure this activity. This continuous, kinetic assay is based on the displacement of fluorescent dyes from dsDNA upon DNA unwinding. These ligands exhibit significant fluorescence enhancement when bound t...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Eggleston, A K, Rahim, N A, Kowalczykowski, S C
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 1996
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC145774/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8614617/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/24.7.1179
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