A helicase assay based on the displacement of fluorescent, nucleic acid-binding ligands.
We have developed a new helicase assay that overcomes many limitations of other assays used to measure this activity. This continuous, kinetic assay is based on the displacement of fluorescent dyes from dsDNA upon DNA unwinding. These ligands exhibit significant fluorescence enhancement when bound t...
Αποθηκεύτηκε σε:
| Εκδόθηκε σε: | Nucleic Acids Res |
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| Κύριοι συγγραφείς: | , , |
| Μορφή: | Artigo |
| Γλώσσα: | Inglês |
| Έκδοση: |
Oxford University Press
1996
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| Θέματα: | |
| Διαθέσιμο Online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC145774/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8614617/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/24.7.1179 |
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