A helicase assay based on the displacement of fluorescent, nucleic acid-binding ligands.

We have developed a new helicase assay that overcomes many limitations of other assays used to measure this activity. This continuous, kinetic assay is based on the displacement of fluorescent dyes from dsDNA upon DNA unwinding. These ligands exhibit significant fluorescence enhancement when bound t...

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Detaylı Bibliyografya
Asıl Yazarlar: Eggleston, A K, Rahim, N A, Kowalczykowski, S C
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 1996
Konular:
Research Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC145774/
https://ncbi.nlm.nih.gov/pubmed/8614617
Etiketler: Etiketle
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