A helicase assay based on the displacement of fluorescent, nucleic acid-binding ligands.

We have developed a new helicase assay that overcomes many limitations of other assays used to measure this activity. This continuous, kinetic assay is based on the displacement of fluorescent dyes from dsDNA upon DNA unwinding. These ligands exhibit significant fluorescence enhancement when bound t...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Eggleston, A K, Rahim, N A, Kowalczykowski, S C
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 1996
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC145774/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8614617/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/24.7.1179
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