Mechanism of origin unwinding: sequential binding of DnaA to double- and single-stranded DNA
The initiator protein DnaA of Escherichia coli binds to a 9mer consensus sequence, the DnaA box (5′-TT(A)/(T)TNCACA). If complexed with ATP it adopts a new binding specificity for a 6mer consensus sequence, the ATP-DnaA box (5′-AGatct). Using DNase footprinting and surface plasmon resonance we show...
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| Udgivet i: | EMBO J |
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| Principais autores: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Nature Publishing Group
2001
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC145534/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11250912/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/emboj/20.6.1469 |
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