Spectrophotometric assay for enzyme-mediated unwinding of double-stranded DNA.
A method is described for monitoring the enzyme-mediated unwinding of duplex DNA spectrophotometrically. The assay employs a partially duplex oligonucleotide substrate modified at the complementary end with coumarin and fluorescein moieties. When in close proximity the fluorescein quenches the fluor...
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| Pubblicato in: | Proc Natl Acad Sci U S A |
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| Autori principali: | , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
National Academy of Sciences
1994
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC44017/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8202510/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.91.12.5471 |
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