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Mechanism of origin unwinding: sequential binding of DnaA to double- and single-stranded DNA
The initiator protein DnaA of Escherichia coli binds to a 9mer consensus sequence, the DnaA box (5′-TT(A)/(T)TNCACA). If complexed with ATP it adopts a new binding specificity for a 6mer consensus sequence, the ATP-DnaA box (5′-AGatct). Using DNase footprinting and surface plasmon resonance we show...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2001
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC145534/ https://ncbi.nlm.nih.gov/pubmed/11250912 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/emboj/20.6.1469 |
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