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Improvement of Posttranslational Bottlenecks in the Production of Penicillin Amidase in Recombinant Escherichia coli Strains

Using periplasmic penicillin amidase (PA) from Escherichia coli ATCC 11105 as a model recombinant protein, we reviewed the posttranslational bottlenecks in its overexpression and undertook attempts to enhance its production in different recombinant E. coli expression hosts. Intracellular proteolytic...

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Detalhes bibliográficos
Main Authors: Ignatova, Z., Mahsunah, A., Georgieva, M., Kasche, V.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2003
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC143610/
https://ncbi.nlm.nih.gov/pubmed/12571052
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.2.1237-1245.2003
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