Overproduction of Thermus sp. Strain T2 β-Galactosidase in Escherichia coli and Preparation by Using Tailor-Made Metal Chelate Supports

A novel thermostable chimeric β-galactosidase was constructed by fusing a poly-His tag to the N-terminal region of the β-galactosidase from Thermus sp. strain T2 to facilitate its overexpression in Escherichia coli and its purification by immobilized metal-ion affinity chromatography (IMAC). The pol...

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Main Authors: Pessela, Benevides C. C., Vian, Alejandro, Mateo, César, Fernández-Lafuente, Roberto, García, José L., Guisán, José M., Carrascosa, Alfonso V.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2003
Assuntos:
Enzymology and Protein Engineering
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC154830/
https://ncbi.nlm.nih.gov/pubmed/12676671
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.4.1967-1972.2003
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