Overproduction of Thermus sp. Strain T2 β-Galactosidase in Escherichia coli and Preparation by Using Tailor-Made Metal Chelate Supports

A novel thermostable chimeric β-galactosidase was constructed by fusing a poly-His tag to the N-terminal region of the β-galactosidase from Thermus sp. strain T2 to facilitate its overexpression in Escherichia coli and its purification by immobilized metal-ion affinity chromatography (IMAC). The pol...

Полное описание

Библиографические подробности

Опубликовано в::	Appl Environ Microbiol
Главные авторы:	Pessela, Benevides C. C., Vian, Alejandro, Mateo, César, Fernández-Lafuente, Roberto, García, José L., Guisán, José M., Carrascosa, Alfonso V.
Формат:	Artigo
Язык:	Inglês
Опубликовано:	American Society for Microbiology (ASM) 2003
Предметы:	Enzymology and Protein Engineering
Online-ссылка:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC154830/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12676671/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.69.4.1967-1972.2003
Метки:	Добавить метку Нет меток, Требуется 1-ая метка записи!