Structure of the β-Galactosidase Gene from Thermus sp. Strain T2: Expression in Escherichia coli and Purification in a Single Step of an Active Fusion Protein

The nucleotide sequence of both the bgaA gene, coding for a thermostable β-galactosidase of Thermus sp. strain T2, and its flanking regions was determined. The deduced amino acid sequence of the enzyme predicts a polypeptide of 645 amino acids (M(r), 73,595). Comparative analysis of the open reading...

Полное описание

Библиографические подробности

Опубликовано в::	Appl Environ Microbiol
Главные авторы:	Vian, Alejandro, Carrascosa, Alfonso V., García, José L., Cortés, Estrella
Формат:	Artigo
Язык:	Inglês
Опубликовано:	American Society for Microbiology (ASM) 1998
Предметы:	Enzymology and Protein Engineering
Online-ссылка:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC106297/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9603833/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.64.6.2187-2191.1998
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